Antithrombin Xa

REF: 5502, 5507

Intended use

The Antithrombin Xa kit is a chromogenic assay intended for the quantitative determination of Antithrombin III (AT-lll) activity in human citrated plasma. This two-stage method utilises Factor Xa, added to a patient plasma dilution containing AT-lll in the presence of excess heparin and calcium. After an initial incubation period (stage 1), residual Factor Xa is determined with a Factor Xa-specific chromogenic substrate (stage 2). The residual Factor Xa activity is inversely proportional to the AT-lll concentration.

Main features

  • Factor Xa assay
  • Insensitive to Heparin Cofactor II
  • Linear calibration from 7.5 - 150%
  • Excellent open vial stability

Kit contents

Component

REF

Volume

Stability

Factor Xa Substrate

5502
5507

3 × 10 mL
5 × 2 mL

Once reconstituted:
2 months at +2 –+6°C
1 month at +17°C

2 days at +37°C

Factor Xa

5502
5507

3 × 10 mL
5 × 2 mL

Once reconstituted:
2 months at +2 –+6°C
1 month at +17°C

7 days at +37°C

Sample Diluent

5502
5507

4 × 10 mL
5 × 3 mL

Store at +2 –+6°C
once opened and use within
1 month


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